Phantom another being and materialization of DNA molecules. Experiments.
Winner of the Nobele Award 2008., for their discovery of human immunodeficiency virus, Luc Montagnier and sponsors (Jamal Aissa, Claude Lavallee, Luc Montagnier ) patented method “teleportation” DNA www.google.com/patents/WO2012142568A2?cl = en , then, l. Montagnier published a article about this http://www.rexresearch.com/montagnier/montagnier.pdf
This work has caused a stormy, mostly negative, reactions in the scientific world, as it turns our perceptions of the roles of DNA and confronts the task of explaining this phenomenon physicists. The method is complicated and not actually Montagnier discovered in detail.
We have developed a much more simple and easily reproducible method of quantum transmission of copies of DNA based on laser technology, that is confirmed by PCR.
Publishing perspectives working genes on the basis of such technology are vast. First of all, This programming stem cells. This gives a real opportunity to regenerate the retina – return the vision (the precedent of this we have created), regenerate teeth (the precedent of this we have created), generally all the organs and tissues, endocrine glands, to eliminate damage to the spinal cord and brain (the precedent of this we have created), shoot the chromosomal damage, for example, in cystic fibrosis (the precedent of this we have created), turn off the extra chromosome in down’s syndrome (the precedent of this we have created), treat the terminal stage of cancer (We have done). And much more. This is potentially a huge business.
Further work in this direction will yield even more powerful impetus to the development of biology, biokomp′ûtingu, medicine, Agriculture, biointernetu, deep space communications, etc. d. We have published some theoretical analysis of this phenomenon, We actually received long before the work of l. Montagnier — based on the example of quantum transmission running complex genes to regenerate the pancreas in rats [Garyaev P., Kokaya A., Mukhina I., Leonova-Garyaeva E., Кокая Н. Г., 2007, Effect of modulated electromagnetic radiation on biostrukturami for alloksanovogo of diabetes mellitus in rats. Бюллетень Эксп. Биол. и Мед., # 2, с. 155-158], http://vixra.org/pdf/1111.0107v1.pdf , http://dnadecipher.com/index.php/ddj/article/view/4 , http://www.tgdtheory.fi/curri.html L.Montane group experiments nobody played, because the key points method not revealed even in the patent.
Now it is time to reproduce the experiments of Luc Montagnier, but in a different way, using our laser technology. We have implemented this. Significantly, It was discovered by a whole new set of phenomena, accompanying the „teleportation” quantum equivalent of DNA in water. Deliberately giving these primary results without parts and without discussion. Early. Main, What we have also demonstrated the possibility of PCR DNA synthesis on a purely aqueous matrix, which recorded the wave equivalent of DNA in the form of a DNA fragment of the modulated wideband electromagnetic field (MBER). This is a secondary electromagnetic field used by us laser. The theory of education of secondary field is given in our article [I.v. Prangishvili, P.P. Garyaev, G.g. Dmitri Tertyshny, Vladimir Maksimenko, A.v. Mologin, E. Leonova, E.r. Ernest. Sensors and systems, # 9 (18), c. 2-13 (2000g.) SPECTROSCOPY OF LOCALIZED RADIATION PHOTON-WAVE: ACCESS TO NON-LOCAL QUANTUM BIO-INFORMATIONAL PROCESSES].
But, Once again, did we not, How did a group of Luc Montagnier.
Here is the result of our experiments on the synthesis of DNA fragment in his Phantom. Let Me Remind you, DNA phantoms I managed to detect and register in 1984. Details in monograph Gariaev P.p., 2009g.,
. Kiev. 220s.
Methods for Amplification. Phantom 547 bp long DNA. with Polymerase Chain Reaction (PCR) Using Modulated Secondary Broadband Electromagnetic Radiation (MBER) of LGN-303 Laser as a Quantum Equivalent of Analogous Material DNA .
For a complete description of the method of detection and materialization of phantoms DNA PCR System.
Step 1. Getting the original DNA product
The PCR product of 547 bp was taken as a starting DNA product, for the receipt of which used a cloned in plazmidnom vector synthetic sequence.
Plus a chain of DNA:
For PCR amplification of DNA-based product used a pair of primers:
Each PCR mixture was 25 ul final volume contained: 67 mM Tris-HCl pH 8,6 at 25 ° C; 2,5 mM magnesium chloride; 16,6 mM ammonium sulfate; dNTPs mixture at a total concentration of 300 uM; primer mix to 0,5 uM each; 2,5 units. Taq DNA polymerase and plasmid DNA template of 25 ng. PCR temperature control included:
initial denaturation at 94° c – 3 min.;
30 cycles: 94 ° C – 30,, 62 ° C – 30,, 72 ° C – 40 with;
final synthesis at 72 ° C for 5 minutes.
PCR- the product was purified from primers and PCR reaction components by using the cleaning kit using SiO2-magnetic particles („Silex”, Russia) in accordance with the manufacturer’s recommendations. We are using 10 l of magnetic particles with binding capacity 10 ug DNA. The elution of DNA was performed in a volume of 50 ul of elution buffer.
step 2. Getting mŠÈI spectrum DNA product.
A drop of the aqueous PCR product solution in a volume of 25 ul was applied to a clean microscope slide and used to probe beam HeNe laser LGN-303 3 min. And above. Received secondary modulated broadband electromagnetic radiation (MBER) recorded transistor radio at a frequency of 700 kHz and converted into sound wave format. This audio file, we suggest you use to introduce DNA information (without the use of, This version of the, Laser LGN-303) in the samples sterile water, having in mind, that sound can carry torsion http information://www.efir.com.ua/rus/a.php?r = 2&d = 69 , including DNA (hypothesis).
At a distance of 15-20 cm from the laser tripod with tubes placed, containing purified distilled water without impurities DNA, RNA and nukleaz. The water was previously frozen at -20° c and razmorožena at room temperature (melt water).
step 3. PCR amplification using samples of water, processed mŠÈI range of original DNA, which reading information laser LGN-303
After exposure to laser water, processed mŠÈI range, used for the production of standard PCR reactions, 25 .mu.l in volume without adding real template DNA. Tripod tubes placed at a distance of 20-30 cm from the laser. The placing of PCR reactions carried out in sterile box, processed UV light, which prevents contamination of samples.
Each PCR mixture contained: 67 mM Tris-HCl pH 8,6 at 25 ° C; 2,5 mM magnesium chloride; 16,6 mM ammonium sulfate; dNTPs mixture at a total concentration of 300 uM; primer mix to 0,5 uM each; 2,5 units. Taq DNA polymerase. Used several temperature PCR, differing duration phase of elongation (synthesis) strands of DNA at 72° c.
The original PCR temperature control included:
initial denaturation at 94 ° C – 3 min.;
40 cycles: 94 ° C – 30,, 62 ° C – 30,, 72 ° C – 40 with;
final synthesis at 72 ° C for 5 minutes.
Modified PCR temperature control included:
initial denaturation at 94 ° C – 3 min.;
40 cycles: 94 ° C – 30,, 62 ° C – 30,, 72 ° C – 2-7 min.;
final synthesis at 72 ° C for 5-7 minutes.
All temperature regimes led to the synthesis of PCR product specified length, but to varying degrees,. The largest number of test specimens of the expected product was observed using a 7-minute DNA strand synthesis in each of 40 PCR cycles.
step 4. Analysis of results of PCR
After the completion of the PCR reaction samples mixed with buffer for gel, containing fluorescent dye SYBR Green I („Silex”, Russia) and analyzed on a 1,5% agarose gel by standard methods of gel electrophoresis in TBE buffer single. Results were analyzed on a transilluminator at 365 nm. Positive thought samples, bands which were at a similar distance from the start, that and the Strip positive control, appropriate size DNA fragment of 547 bp.
Experimental samples were subjected to sequencing, positive control samples PCR and DNA samples, source product, with which made reading laser. Sequencing revealed, The experimental samples 99,2-100% identical to the DNA sequence of the initial product, which made reading laser.
As an illustration of one of our experiments, see picture PCR DNA phantoms. Sound recording mŠÈI DNA in wave format on the frequency 700kGc also provides.
PS: Our experiments, you can play without using laser. And without the original DNA matrix (as we also do), i.e.. do not synthesize matrix. This ensures against accidental closure of the original DNA in working with water tubes, the pipettes, etc.. Thus we go from kontaminacij. In this embodiment, the audio player working set at a distance from 1 to 20 cm from the test tubes with the tripod. Exposure time may vary between 5 minutes and up. Identity formed DNA PCR product and the original DNA sequencing DNA obtained can be set. In this version of the test enough primers and knowledge consistency
original DNA nucleotides.
Dr. Peter P. Garyaev.
D.b.s., ACAD.. RANS, RAMTN and LIST, TSP. New York EN.
Scientific Director of the Institute of quantum Genetics